Cloned (Comment) | Organism |
---|---|
gene proS, expression in Escherichia coli strain BL21(DE3) | Methanothermobacter thermautotrophicus |
gene proS, expression in Escherichia coli strain BL21(DE3) | Methanocaldococcus jannaschii |
gene proS, expression in Escherichia coli strain SG13009 | Methanococcus maripaludis |
gene proS, overexpression of His-tagged wild-type ProRS and truncation mutant ScDELTA183 | Saccharomyces cerevisiae |
gene proS, overexpression of wild-type and mutant His-tagged ProRS | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a truncated Sc ProRS mutant lacking the N-terminal 183 residues, ScDELTA183, which shows reduced enzyme activity compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
additional information | replacement of 163 residues of the INS domain, amino acids 232-394, with either an 8-residue Gly6Ser2 linker or a 16-residue Gly12Ser4 linker by PCR amplification of the full-length plasmid pCS-M1S | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-O-[N-(L-alanyl)-sulfamoyl]adenosine | a non-hydrolyzable adenylate analogue, a potent inhibitor of the ATP-diphosphate exchange reaction | Escherichia coli | |
5'-O-[N-(L-Prolyl)-sulfamoyl]adenosine | a non-hydrolyzable adenylate analogue | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000131 | - |
tRNAPro | pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, aminoacylation | Escherichia coli | |
0.01414 | - |
tRNAPro | pH 7.0, 37°C, recombinant wild-type enzyme, aminoacylation | Escherichia coli | |
0.25 | - |
L-proline | pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation | Escherichia coli | |
2 | 37 | trans-4-hydroxyproline | pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation | Escherichia coli | |
50 | - |
L-proline | pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, amino acid activation | Escherichia coli | |
53 | - |
cis-4-hydroxyproline | pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, amino acid activation | Escherichia coli | |
55 | - |
cis-4-hydroxyproline | pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation | Escherichia coli | |
140 | - |
L-alanine | pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Saccharomyces cerevisiae | |
Mg2+ | - |
Methanothermobacter thermautotrophicus | |
Mg2+ | - |
Methanocaldococcus jannaschii | |
Mg2+ | - |
Methanococcus maripaludis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | Escherichia coli | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | Methanothermobacter thermautotrophicus | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | Methanococcus maripaludis | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Methanocaldococcus jannaschii | - |
gene proS | - |
Methanococcus maripaludis | - |
gene proS | - |
Methanothermobacter thermautotrophicus | - |
gene proS | - |
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
additional information | - |
- |
Methanothermobacter thermautotrophicus |
additional information | - |
- |
Methanocaldococcus jannaschii |
additional information | - |
- |
Methanococcus maripaludis |
additional information | - |
activities of wild-type and mutant enzymes, pre-transfer editing activity, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cis-4-hydroxyproline + tRNAPro | - |
Escherichia coli | AMP + diphosphate + cis-4-hydroxyprolyl-tRNAPro | - |
? | |
ATP + L-alanine + tRNAPro | L-alanine is a poor substrate | Escherichia coli | AMP + diphosphate + L-alanyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Escherichia coli | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Methanococcus maripaludis | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | editing mechanism, the aminoacylation active site plays a significant role in preserving the fidelity of translation by acting as a filter that selectively releases non-cognate adenylates into solution, while protecting the cognate adenylate from hydrolysis, overview, scheme showing proposed pre-transfer and posttransfer editing pathways, overview | Escherichia coli | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + trans-4-hydroxyproline + tRNAPro | - |
Escherichia coli | AMP + diphosphate + trans-4-hydroxyprolyl-tRNAPro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class II prolyl-tRNA synthetase | - |
Escherichia coli |
class II prolyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
class II prolyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
class II prolyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
class II prolyl-tRNA synthetase | - |
Methanococcus maripaludis |
Prolyl-tRNA synthetase | - |
Escherichia coli |
Prolyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
Prolyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
Prolyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-tRNA synthetase | - |
Methanococcus maripaludis |
ProRS | - |
Escherichia coli |
ProRS | - |
Saccharomyces cerevisiae |
ProRS | - |
Methanothermobacter thermautotrophicus |
ProRS | - |
Methanocaldococcus jannaschii |
ProRS | - |
Methanococcus maripaludis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
37 | - |
assay at | Methanothermobacter thermautotrophicus |
37 | - |
assay at | Methanocaldococcus jannaschii |
37 | - |
assay at | Methanococcus maripaludis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0091 | - |
tRNAPro | pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, aminoacylation | Escherichia coli | |
0.056 | - |
cis-4-hydroxyproline | pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, amino acid activation | Escherichia coli | |
0.239 | - |
tRNAPro | pH 7.0, 37°C, recombinant wild-type enzyme, aminoacylation | Escherichia coli | |
1.7 | - |
L-alanine | pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation | Escherichia coli | |
12 | - |
L-proline | pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, amino acid activation | Escherichia coli | |
15 | - |
trans-4-hydroxyproline | pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation | Escherichia coli | |
21 | - |
cis-4-hydroxyproline | pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation | Escherichia coli | |
70 | - |
L-proline | pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Saccharomyces cerevisiae |
7 | - |
assay at | Methanothermobacter thermautotrophicus |
7 | - |
assay at | Methanocaldococcus jannaschii |
7 | - |
assay at | Methanococcus maripaludis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | - |
Saccharomyces cerevisiae | |
ATP | - |
Methanothermobacter thermautotrophicus | |
ATP | - |
Methanocaldococcus jannaschii | |
ATP | - |
Methanococcus maripaludis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000043 | 0.000088 | 5'-O-[N-(L-alanyl)-sulfamoyl]adenosine | ATP-diphosphate exchange reaction, pH 7.0, 37°C, recombinant wild-type enzyme | Escherichia coli |